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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
20/09/2019 |
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Data da última atualização: |
18/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TRICHEZ, D.; STEIDORFF, A. S.; SOARES, C. E. V. F.; FORMIGHIERI, E. F.; ALMEIDA, J. R. M. de. |
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Afiliação: |
Débora Trichez, bolsista da Embrapa Agroenergia.; Andrei S. Steindorff, U.S. Department of Energy (DOE) Joint Genome Institute.; Carlos E. V. F. Soares, UnB; EDUARDO FERNANDES FORMIGHIERI, CNPAE; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE. |
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Título: |
Physiological and comparative genomic analysis of new isolated yeasts Spathaspora sp. JA1 and Meyerozyma caribbica JA9 reveal insights into xylitol production. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
FEMS Yeast Research, v. 19, 2019. |
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Páginas: |
15 p. |
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Idioma: |
Inglês |
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Conteúdo: |
Xylitol is a five-carbon polyol of economic interest that can be produced by microbial xylose reduction from renewable resources. The current study sought to investigate the potential of two yeast strains, isolated from Brazilian Cerrado biome, in the production of xylitol as well as the genomic characteristics that may impact this process. Xylose conversion capacity by the new isolates Spathaspora sp. JA1 and Meyerozyma caribbica JA9 was evaluated and compared with control strains on xylose and sugarcane biomass hydrolysate. Among the evaluated strains, Spathaspora sp. JA1 was the strongest xylitol producer, reaching product yield and productivity as high as 0.74 g/g and 0.20 g/(L.h) on xylose, and 0.58 g/g and 0.44 g/(L.h) on non-detoxified hydrolysate. Genome sequences of Spathaspora sp. JA1 and M. caribbica JA9 were obtained and annotated. Comparative genomic analysis revealed that the predicted xylose metabolic pathway is conserved among the xylitol-producing yeasts Spathaspora sp. JA1, M. caribbica JA9 and Meyerozyma guilliermondii, but not in Spathaspora passalidarum, an efficient ethanol-producing yeast. Xylitol-producing yeasts showed strictly NADPH-dependent xylose reductase and NAD+-dependent xylitol-dehydrogenase activities. This imbalance of cofactors favors the high xylitol yield shown by Spathaspora sp. JA1, which is similar to the most efficient xylitol producers described so far. |
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Palavras-Chave: |
Bagasse hydrolysate; Comparative genomics; Spathaspora; Xylose fermentation. |
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Thesaurus Nal: |
Meyerozyma; Xylitol. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02207naa a2200253 a 4500
001 2112351
005 2019-11-18
008 2019 bl uuuu u00u1 u #d
100 1 $aTRICHEZ, D.
245 $aPhysiological and comparative genomic analysis of new isolated yeasts Spathaspora sp. JA1 and Meyerozyma caribbica JA9 reveal insights into xylitol production.$h[electronic resource]
260 $c2019
300 $a15 p.
520 $aXylitol is a five-carbon polyol of economic interest that can be produced by microbial xylose reduction from renewable resources. The current study sought to investigate the potential of two yeast strains, isolated from Brazilian Cerrado biome, in the production of xylitol as well as the genomic characteristics that may impact this process. Xylose conversion capacity by the new isolates Spathaspora sp. JA1 and Meyerozyma caribbica JA9 was evaluated and compared with control strains on xylose and sugarcane biomass hydrolysate. Among the evaluated strains, Spathaspora sp. JA1 was the strongest xylitol producer, reaching product yield and productivity as high as 0.74 g/g and 0.20 g/(L.h) on xylose, and 0.58 g/g and 0.44 g/(L.h) on non-detoxified hydrolysate. Genome sequences of Spathaspora sp. JA1 and M. caribbica JA9 were obtained and annotated. Comparative genomic analysis revealed that the predicted xylose metabolic pathway is conserved among the xylitol-producing yeasts Spathaspora sp. JA1, M. caribbica JA9 and Meyerozyma guilliermondii, but not in Spathaspora passalidarum, an efficient ethanol-producing yeast. Xylitol-producing yeasts showed strictly NADPH-dependent xylose reductase and NAD+-dependent xylitol-dehydrogenase activities. This imbalance of cofactors favors the high xylitol yield shown by Spathaspora sp. JA1, which is similar to the most efficient xylitol producers described so far.
650 $aMeyerozyma
650 $aXylitol
653 $aBagasse hydrolysate
653 $aComparative genomics
653 $aSpathaspora
653 $aXylose fermentation
700 1 $aSTEIDORFF, A. S.
700 1 $aSOARES, C. E. V. F.
700 1 $aFORMIGHIERI, E. F.
700 1 $aALMEIDA, J. R. M. de
773 $tFEMS Yeast Research$gv. 19, 2019.
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Embrapa Agroenergia (CNPAE) |
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| 1. |  | FERREIRA, G. B.; MOREIRA, M. M.; CHAVES, V. C.; SILVA, M. S. L. da; COSTA, M. B. B. da; ALVES, C. de A.; MEIRA, R. A. A organização do trabalho em agroecossistemas com barragens subterrâneas no semiarido brasileiro. Cadernos de Agroecologia, v. 4, n. 1, p. 3669-3672, 2009. Trabalho apresentado no 6º Congresso Brasileiro de Agroecologia e 2º Congresso Latinoamericano de Agroecologia, 2009, Curitiba.| Tipo: Artigo em Anais de Congresso |
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| 2. | | OLIVEIRA, T. P. de; PACHECO, C. S. G. R.; OLIVEIRA, L. M. S. R. de; NASCIMENTO, J. A. do; VIEIRA, D. D.; SILVA, A. F.; MARINHO, C. M.; SANTIAGO, F. dos S.; SCHEPP, A. M. dos S.; MEIRA, R. A. Organismos participativos de avaliação da conformidade orgânica e oportunidade para acesso a mercados em rede: experiência do Projeto algodão em consórcios agroecológicos. In: PACHECO, C. S. G. R.; OLIVEIRA, L. M. S. R. de; OLIVEIRA, L. S. de; NASCIMENTO JÚNIOR, J. A.; SANTOS, R. P. dos; SANTOS, H. dos R. (org.). Extensão rural: desafios e perspectivas para o fortalecimento de práticas agrícolas sustentáveis. Guarujá: Científica Digital, 2022. Cap. 21, p. 281-294.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Semiárido. |
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